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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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All mice were housed in a specific pathogen-free animal facility in individually ventilated cages under controlled conditions (23 ± 3 °C, 50% ± 10% humidity) with a 12-h light/dark cycle (light: 07:00 to 19:00

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