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3.2.3 Immunocytochemical studies reveal co-localization of HPX with both TfR1 and LRP1 and also the separation of TfR and LRP1 trafficking in human hepatoma HepG2 cells 3.2.3.1 Rationale for using human hepatoma HepG2 cells Liver targeting of heme-HPX was observed after intravenous injection of heme–HPX in anesthetized rats (Smith and Morgan, 1978)

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In brief, cells were washed once with PBS and lysed by the addition of luciferase lysis buffer (1% Triton X-100, 25 mM Glycyl-Glycin (pH 7.8), 15 mM MgSO 4 , 4 mM EGTA, 1 mM DTT)