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1 Introduction 1.1 Historical background Since its discovery, the process of RNA interference (RNAi), as a method of gene silencing, has attracted keen interest for applications in medicine ( 1.2 Core mechanisms and pathways: the siRNA pathway as an antiviral defense mechanism In contrast to mammals, but similarly to plants, insects possess a separate machinery for processing of long dsRNAs into small RNAs ( 1.3 Early uses and concepts of RNAi in pest control Because of the feature of intracellular defense against viral infection, the process of RNAi is considered to be very efficient in all insects once the delivery in the cytoplasm occurs ( 1.4 Integration of the RNAi mechanism in a broader view of the regulation of innate immunity In model organisms such as Drosophila and mosquitoes, recent research has revealed the importance of dsRNA as a trigger of innate immunity pathways, beyond the RNAi mechanism