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The assay readout is the proportion of PCNA-positive nuclei among total counted cells, but PCNA labeling is not identical to BrdU labeling because PCNA can mark late G1/early S-associated replication competence and may persist beyond active DNA synthesis depending on fixation and extraction conditions
liquid
Syringe:29G
In this model, successful repair proceeds through natural reprogramming of epithelial cells to a more embryonic-like state, marked by the murine stem cell marker Ly6a (encoding SCA1) 22,59,60
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