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108 H289 in Cre recombinase has been shown to be unable to accommodate bulky aromatic residues, making it an attractive choice for NPOMH introduction to demonstrate the utility of the ncAA to block protein activity due to steric hindrance imposed on an active site

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In further studies, only the formation of [14C]carbon dioxide from [14C-1]glucose was measured, with the addition of: sodium ascorbate (which undergoes a non-enzymic reaction in air to produce hydrogen peroxide) acetylphenylhydrazine (which is known to precipitate haemolysis in sensitive subjects, and depletes glutathione) methylene blue (which oxidises NADPH) All incubations were repeated in the presence and absence of N -ethylmaleimide, which undergoes a non-enzymic reaction with the -SH group of reduced glutathione, and thus depletes the cell of glutathione