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In uncomplicated KT, high blood dd-cfDNA levels are encountered, with a median value of approximately 20% immediately (within hours) after renal engraftment and rapidly decreases on the first postoperative day to approximately 5% and then subsequently to below 1% ( According to a meta-analysis, the sensitivity for AMR diagnosis is high at a fractional threshold of 1%, but less sensitive for TCMR, which generally needs a concentration higher than a 1% threshold, especially if the rejection is more severe than Banff 1B ( Notably, most of the current studies on dd-cfDNA are ad hoc tests on patients who probably have a high pre-test risk of rejection