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Despite the strength of findings in SH-SH5Y cells, it is imperative to determine how D-CYSee and betaine affect chronic morphine-induced changes in redox (e.g., GSH, GSSG) and epigenetic signatures of physical dependence in brain regions involved in development of physical dependence to opioids, such as medial prefrontal cortex (MPFC), striatum and hippocampus (Deslandes et al., 2002

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