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Lean Lab India
Batches of 100 size-matched islets transfected with adenovirus expressing control or Vgf -shRNA were preincubated in KRBH buffer containing 0.5% BSA and 7 mM glucose at 37 °C for 30 min
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For additional examination, the supernatant was kept at −80°C after the homogenate was centrifuged at 10,000 g for 15 min at 4°C
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