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Thus, U373 and HMC3 cells were treated with Fer-1 (10 μM) or the vehicle (DMSO), followed by infection with HSV-1 (MOI = 0.1) for 24 h

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Formation of GSH conjugates can be detected by mass spectrometry, which, in turn, provides insight into the reactive metabolite structure.96,97 With the possible exception of acyl glucuronides and cyclic iminium ions, most reactive metabolites are generally short-lived and are not usually detectable in circulation