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Through specific knockdown of the NPY receptor (NPY2R or NPY4R) achieved by siRNA transfection, combined with techniques such as Western blot (WB), ELISA and RT-qPCR, we found that NPY primarily activated the NF-κB signaling pathway via the NPY2R, thereby inducing the expression of key inflammatory cytokines, including CXCL9, CXCL10, and IL-15
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