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Effect of DMSO on the Proliferation of Selected Cell Lines To examine the effect of DMSO (the concentrations range 1–10%) on the proliferation of human colorectal adenocarcinoma (Caco-2) cells and on the murine model of mucopolysaccharidosis, type IIIB, i.e., the WT (the control) and Naglu −/− (the line with mutation in gene encoding N-alpha-acetylglucosaminidase) cell lines, the cells were treated with DMSO using three different ways of administration: (1) directly to the medium culture supplemented with serum (Figure 2A)

where they are converted to usable energy