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Background fluorescence due to spatial disparities and endogenous fluorescence were removed using Fiji to detect the mean fluorescent intensity of Alexa Fluor 488 dextran in the median eminence and ARC sections
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The observed prescriptions count in 2024 was 2 230 729 versus 1 509 901 predicted (95% CI: 1 465 348 to 1 554 454)
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