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The sequences of primers were as follows: TNF-α (5′-CTG TAG CCC ACG TCG TAG CAA-3′ and 5′-TGT CTT TGA GAT CCA TGC CGT T-3′), IL-6 (5′-ATA GTC CTT CCT ACC CCA ATT TCC-3′ and 5′-GAT GAA TTG GAT GGT CTT GGT CC-3′), and GAPDH (5′-AGA ACA TCA TCC CTG CAT CCA C-3′ and 5′-TCA GAT CCA CGA CGG ACA CA-3′) 2.16