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Western Blotting To evaluate NGF levels in the striatum and for phosphorylation levels, fresh tissue was homogenized at a 30% ratio (w v −1 ) in kinase extraction buffer A (10 mM HEPES, pH 7.9, 10 mM KCl, 10 mM EDTA, 1 mM DTT, 0.4% v v −1 IGEPAL, 1 mM Na 3 VO 4 , 1 mM PMSF, and 10 mg mL −1 aprotinin and leupeptin) using a previously described method (Kaneko et al., 2004)