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After blocking with 5% nonfat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 1 h at room temperature, membranes were incubated overnight at 4 °C with the following primary antibodies: LYVE-1 (1:500, ab219556, rabbit monoclonal, Abcam), p-PI3K (p85, Tyr458
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